Nova Scotia Duck Tolling Retriever ONLY


Buff is a recessively inherited coat color variant that occurs in the NSDTR. Buff is a disqualifying feature for the show ring but otherwise appears to cause no health issues in the breed. Puppies are born a lighter shade of red that can appear silver in color. As they age they can sometimes darken to a more red color but they are lighter than they would be without the effect of the buff mutation.nsdtr

Buff coat color is caused by mutations in the gene called melanophilin which also causes dilute coat color in many other breeds (Drögemüller C, U. Philipp, B. Haase, A-R Günzel-Apel T Leeb. A noncoding melanophilin gene (MLPH) SNP at the splice donor of exon 1 represents a candidate causal mutation for coat color dilution in dogs. Journal of Heredity (2007) 98(5):468-473). The mutation that causes buff is the same exact change in the DNA sequence that causes dilute.

The Mode of Inheritance

This coat color is inherited as an autosomal recessive disease meaning that affected puppies inherited two mutant copies of this gene, one from each of their parents. 

The Test Results

BUFF N/N: This dog has two normal copies of the BUFF gene
BUFF N/A: This dog has one mutant (abnormal) copy of the BUFF gene
BUFF A/A: This dog has two mutant (abnormal) copies of the BUFF gene


BUFF is inherited as an autosomal recessive disease meaning that animals that are N/A are normal colored but can produce buff colored puppies if bred to a buff colored dog (A/A) or another carrier (N/A). Buff carriers will produce 50% carrier puppies.

Order DNA Test

Tests are ordered online through the secure area of the OFA website. Payment is accepted by credit card (MasterCard and VISA). The OFA administers all order handling. Upon receipt of an order, the OFA will send out the test kit which will include a Foam-Tipped Applicator card for DNA sample collection, along with sample collection instructions. Using the FTA card technology, owners can safely collect DNA samples at home. The collection process is non-invasive, and no veterinary appointment is necessary.

Samples are then sent to the University of Missouri College of Veterinary Medicine where the samples will be processed by the Small Animal Molecular Genetics Lab. Results will be forwarded to the OFA, and the OFA will issue the resulting report to the owner.

The fee for each test includes the test kit, laboratory processing, and subsequent registration in the OFA databases.